![]() To change the data-collection parameters for your experiment, choose Data Collection from the Experiment menu and make the necessary changes. The default settings are 1 sample per second for 200 seconds.See the previous section for more details. Click to continue or click and select a wavelength on the graph or in the list of wavelengths. The wavelength of maximum absorbance will be selected. ![]() A helper box will appear, displaying the absorbance and concentration of the unknown. Choose Interpolation Calculator from the Analyze menu. If doing Beer’s law to determine the concentration of an unknown, place the unknown sample in the cuvette holder.Click Linear Fit,, to see the best fit line equation for the standard solutions.When finished, click to end data collection. Repeat Step 6 for the remaining samples.Enter the concentration of the second sample and click. Place your second sample in the cuvette slot.Enter the concentration of the sample and click. Place your first sample in the cuvette slot of the Spectrophotometer. Select boxes in the list or drag your cursor on the graph to select up to ten contiguous wavelengths. Option 3 If you wish to measure an average over a range of contiguous wavelengths of your choice, change Single 10 nm Band to Individual Wavelengths. ![]() You may then select up to ten wavelengths to measure at the same time.
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